Delve deeper into the epigenome to reveal heritable genetic traits and discover how environmental factors can lead to gene expression changes with our expertise in epigenetic analysis.
Our genomics team can help you to characterize and profile DNA methylation status, and assess chromatin changes using cutting-edge sequencing and array platforms, providing you with expert analysis and interpretation of the data.
Take advantage of our expertise with our wide range of epigenetic services:
5mC and 6mA methylation studies
Eremid provides direct DNA methylation analysis using either PacBio HiFi or Oxford Nanopore sequencing, with ONT also able to resolve RNA methylation, delivering a comprehensive, high-resolution view of epigenetic regulation in cancer.
We use PacBio’s well-established 5-base HiFi sequencing for its ability to detect 5mC directly in native DNA, providing highly accurate CpG methylation profiling across complex and repetitive genomic regions without requiring bisulfite conversion. With the introduction of PacBio’s new SPRQ chemistry, HiFi sequencing has seen further advancements in methylation detection. The update improves 5mC calling accuracy by 10% and, for the first time, enables direct on-instrument detection of 6mA, significantly enhancing its multiomics capabilities. The new 6mA calling feature means PacBio Revio systems can now run the Fiber-seq assay, a method that enables simultaneous detection of DNA sequence, 5mC and chromatin accessibility, providing a wealth of valuable multiomic information in a single assay.
Whole-genome bisulfite and enzymatic-based Methylation sequencing
Our whole-genome bisulfite and enzymatic-based methylation sequencing services detect and quantify epigenetic modifications such as methylated cytosines to reveal modifications involved in gene silencing, chromosome inactivation and more.
Treating genomic DNA with enzymes or sodium bisulfite, converts unmethylated cytosines to uracil, while methylated cytosines are left intact allowing for stringent comparative analysis of methylated nucleotide sites.
Targeted Methylation sequencing (Capture Methyl-seq)
Targeted methylation sequencing offers a cost-effective option for epigenome analysis when whole-genome bisulfite sequencing is not required.
Infinium methylation profiling array
The Illumina Infinium methylation profiling array is an array-based method that offers the perfect, cost-effective sequencing approach to help you reveal portions of the methylome involved in health and disease.
Hi-C sequencing (chromatin conformation)
Hi-C sequencing provides you with the data you need to understand complex chromatin conformations. Crosslinking chromatin with formaldehyde before digesting allows only those DNA fragments that are covalently linked to re-ligate, providing unrivalled insight into the sequence origin and physical location in the genome.
ATAC sequencing from tissue or single-cell (chromatin accessibility)
The assay for transposase-accessible chromatin with sequencing (ATAC sequencing) offers precise insight into chromatin accessibility to help you better understand gene regulation within your research model. ATAC sequencing can be performed on bulk tissue or single cells to suit the needs of your study.
ChipSeq and CUT&RUN sequencing (chromatin accessibility)
For a sequencing based approach to DNA-protein interactions in the epigenome, ChIP-Seq is the ideal solution. With a combination of chromatin immunoprecipitation and next generation sequencing, we help you to rapidly discover the role of the epigenome in relation to your study.
CUT&RUN-sequencing or cleavage under targets and release using nuclease is an antibody-based sequencing method ideal for low abundant samples. As fixation is not required, background noise is reduced eliminating the need for deep sequencing and minimizing the amount of sample required overall.
Find out how our epigenetic services can help you meet your goals
With extensive expertise in AgBiotech, Food and Nutrition, and Human Health, our team can help you find the epigenetic services you need.
